Improving Rigor and Reproducibility
Western Blotting is one of the most commonly used techniques in scientific research. Yet, many researchers utilize this technique in a manner that is not reproducible
Western Blotting Publications in which Dr. Gomes was asked for his expert opinion or as a validation reference
Jan L.A. Voskuil , Anita Bandrowski, A., Begley, C.G., Bradbury, ARM., Chalmers, AD., Gomes, AV., Hardcastle, T., Lund-Johansen, F., Plückthun, A., Roncador, G., Solache, A., Taussig, MJ., Trimmer, J.S., Williams, C., Goodman, S.L. (2020) The Antibody Society’s antibody validation webinar series. mAbs. 12:1, 1794421.
Belisle, C. (2010) Reproducible Laemmli PAGE Performance. Tutorial. Nov. 1, 30:No 19. (Includes Top Ten Tips to Improve Protein Gel Electrophoresis)
Useful Western Blotting References from the Gomes Lab
Mishra M, Tiwari S, Gunaseelan A, Li D, Hammock BD, Gomes AV. (2020) Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP). Electrophoresis, 40(12-13): 1731-1739.
Sander H, Wallace S, Plouse R, Tiwari S, Gomes AV. (2019) Ponceau S waste: Ponceau S staining for total protein normalization. Analytical Biochemistry, 575: 44-53.
Mishra M, Tiwari S, Gomes AV. (2017) Protein purification and analysis: next generation Western blotting techniques. Expert review of proteomics. 14(11):1037-1053.
Gilda JE, Ghosh R, Cheah JX, West TM, Bodine SC, Gomes AV. (2015) Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS One. 19;10(8):e0135392.
Gilda JE, Gomes AV. (2015) Western blotting using in-gel protein labeling as a normalization control: stain-free technology. Methods Mol Biol. 1295:381-91
Ghosh R, Gilda JE, Gomes AV. (2014) The necessity of and strategies for improving confidence in the accuracy of western blots. Expert Rev Proteomics. 11(5):549-60.
Gilda JE, Gomes AV. (2013) Stain-Free total protein staining is a superior loading control to β-actin for Western blots. Anal Biochem.; 440:186-8.