Improving Rigor and Reproducibility
Western Blotting is one of the most commonly used techniques in scientific research. Yet, many researchers utilize this technique in a manner that is not reproducible
Western Blotting Publications in which Dr. Gomes was asked for his expert opinion or as a validation reference
Belisle, C. (2010) Reproducible Laemmli PAGE Performance. Tutorial. Nov. 1, 30:No 19. (Includes Top Ten Tips to Improve Protein Gel Electrophoresis)
Useful Western Blotting References from the Gomes Lab
Mishra M, Tiwari S, Gomes AV. (2017) Protein purification and analysis: next generation Western blotting techniques. Expert review of proteomics. 14(11):1037-1053.
Gilda JE, Ghosh R, Cheah JX, West TM, Bodine SC, Gomes AV. (2015) Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). PLoS One. 19;10(8):e0135392.
Gilda JE, Gomes AV. (2015) Western blotting using in-gel protein labeling as a normalization control: stain-free technology. Methods Mol Biol. 1295:381-91
Ghosh R, Gilda JE, Gomes AV. (2014) The necessity of and strategies for improving confidence in the accuracy of western blots. Expert Rev Proteomics. 11(5):549-60.
Gilda JE, Gomes AV. (2013) Stain-Free total protein staining is a superior loading control to β-actin for Western blots. Anal Biochem.; 440:186-8.